primary antibody against th mouse anti-th 1:1000 immunostar 22941  (ImmunoStar inc)

Journal: World Journal of Diabetes

Article Title: Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring

doi: 10.4239/wjd.v15.i4.758

Figure Lengend Snippet: Adult female offspring born from dams with streptozotocin-induced gestational diabetes mellitus have increased density of sensory and sympathetic nerve fibers innervating the pancreatic acinar cells. A and B: Representative confocal images of calcitonin gene-related peptide (CGRP) expressing small diameter peptidergic sensory nerve fibers; C and F: Significantly greater densities of CGRP + and tyrosine hydroxylase + (TH + ) nerve fibers were found in offspring born from dams with GMD as compared to control offspring; D and E: Enzyme TH expressing postganglionic sympathetic nerve fibers in acinar pancreatic tissue (40 μm-thick) from offspring born of vehicle or streptozotocin injected dams; a P < 0.05 Student t test, n = 5 per group. Data are shown as mean ± SEM. CGRP: Calcitonin gene-related peptide; TH: Tyrosine hydroxylase; VEH: Vehicle; STZ: Streptozotocin.

Article Snippet: Sympathetic nerve fibers were labeled with primary antibody against tyrosine hydroxylase (TH, polyclonal rabbit anti-mouse 1:1000; EMD Millipore; catalog number AB152).

Techniques: Expressing, Injection

Journal: Frontiers in Molecular Neuroscience

Article Title: Liver kinase B-1 modulates the activity of dopamine neurons in the ventral tegmental area and regulates social memory formation

doi: 10.3389/fnmol.2024.1289476

Figure Lengend Snippet: Activation time course of VTA DA neurons after social approach test. (A) Left: Schematic diagram of the social approach test. Two groups of mice (object vs. social) were allowed to freely interact with either a social conspecific or an object, respectively, for 10 min followed by transcardiac perfusion at different time points. Right: Mice in the social group spent significantly more time interacting with the target compared with that of mice in the object group. (B) Left: Representative images showing the activation of DA neurons in the VTA by co-immunofluorescent staining with Tyrosine hydroxylase (TH, red) and c-fos (green). Bar: 25 μm. Right: Quantification of the total number of activated cells (c-fos + ), activated DA neurons (TH + c-fos + ), and the percentage of activated DA neurons. Each group contained 12 sections from 3 mice for all six groups. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with object; ### p < .0.001 compared with 1.5 h. Data are presented with mean ± S.E.M.

Article Snippet: For immunofluorescent staining, the sections were blocked in blocking buffer (1% BSA, 3% donkey serum, and 0.3% Triton X-100 in phosphate-buffered saline) for 1 h at room temperature and incubated with mouse anti-tyrosine hydroxylase (TH) primary antibody (1:400, #MAB318, Millipore, Temecula, CA, USA) and rabbit anti-c-fos primary antibody (1:400, #2250, Cell Signaling Technology, Danvers, MA, USA) overnight at 4°C.

Techniques: Activation Assay, Staining